Introduction to Mendelgen

Mendelgen is an online sequence editing and manipulation tool. It combines all necessary features for your genetic experiments (DNA digests, primer design, translation features) with minimalistic, aesthetically pleasing and intuitive design, easy on the eyes thanks to day/night mode.

To begin, create or upload your first sequence. Mendelgen supports import of .fasta, .dna, .gb, .gbk, .txt sequence formats, thus it is compatible with many instruments you may be using in the lab. Please refer to the guide to optimize your work using hotkeys and proposed workflows.

This tutorial includes all detail about Mendelgen functionality. You will learn all from creating the sequence to using advanced features such as codon optimization, sequence alignment, etc. To better demonstrate how Mendelgen may assist you in experiments, we provide a case study on the example of cloning into a pLentiCRISPR vector

Table of Contents

1. Introduction to Mendelgen
2. Controls Overview
3. Hotkeys
4. Workspace and Viewing Preferences Dashboard
   • The Contents and Panels of the Workspace
   • Sequence and Vector View
   • Zooming in Function
   • Dark Mode
   • Visibility Options
5. Vector Wizard
6. Working With Your Sequence
   • Adding a Sequence
   • Editing a Sequence
   • Copy/Paste a Section of DNA
   • Adding a Feature or a Part
   • Insert a Common Feature
   • Saving  a Sequence
   • Exporting a Sequence
   • Renaming a Sequence
7. Using Mendelgen for DNA Editing (Advanced Features)
   • Simulating a Restriction Digest
   • Designing Primers
   • Sequence Alignment
   • Codon Optimization
   • Translation
8. Advanced Settings
   • Auto-Annotating Features
   • Thermodynamic Settings
   • Adding Additional Enzymes
9. Case Study: Cloning into lentiCRISPR vector
10. Polymerase Chain Reaction
11. Cloning Methods
    1. Restriction Enzyme Cloning
    2. Gibson Assembly
    3. TA Cloning
    4. Gateway Cloning
    5. Golden Gate Cloning (This one is on Medium)
    6. In-Fusion Cloning